This is the first time in Europe that a technique has been implemented on a large scale to prevent the reproduction of the tiger mosquito without the use of chemical insecticides. The test, carried out at the ENEA Casaccia Research Center in Rome in collaboration with Sapienza University and Google (Debug project), lowered the species' ability to produce viable eggs to survive the winter by 88% when compared to a control area. Based on the Incompatible Male Technique, ENEA replaced the natural strain of the Wolbachia bacterium in the tiger mosquito with that of the common mosquito. This resulted in males that mated with wild females, resulting in eggs that failed to hatch. Google is in charge of expanding the population, selecting only males, and transferring them by plane from Miami to the release location. Between July and October 2025, an estimated 1.6 million eggs were released across 53 hectares. The risk criterion of 100 eggs per trap was only exceeded in two of the 16 samplings, compared to 12 in the control area, while egg fertility was close to zero at the end of the season. The males do not bite and seek only females of their own species, to the extent that the WHO considers the approach one of the most effective. This conclusion is even more relevant today: there have been 330 confirmed cases of dengue since the beginning of the year, 59 of which occurred in Italy, with the most recent outbreak reported in Rome on October 2nd.